rabbit antibody to tgfβ3  (Diagnostic BioSystems)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: Increasing the level of cytoskeletal protein Flightless I reduces adhesion formation in a murine digital flexor tendon model

doi: 10.1186/s13018-020-01889-y

Figure Lengend Snippet: Flii Tg/Tg mice adhesions express higher levels of TGFβ1 and lower levels of TGFβ3 than WT mice. ( a ) TGFβ1 expression levels in the tendon adhesions and ( b ) surrounding dermis show significantly elevated expression in Flii Tg/Tg mice compared with WT mice. ( c ) TGFβ3 expression levels in the tendon adhesions and ( d ) surrounding dermis show significantly elevated expression in WT mice compared with Flii Tg/Tg mice. Data represented as mean ± SEM. * p ≤ 0.05, ** p ≤ 0.01. n = 6

Article Snippet: Following antigen retrieval, 3% normal goat serum diluted in PBS was used for blocking for 30 min. Primary antibodies were used at 2 μg/ml and included mouse α-Flii (Santa Cruz sc-21716), rabbit α-Collagen I (Rockland 600-401-103), rabbit α-Collagen III (Rockland 600-403-105), mouse α-TGFβ1 (Santa Cruz sc-52893) and rabbit α-TGFβ3 (Santa Cruz sc-83).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Regulation of the Epithelial Adhesion Molecule CEACAM1 Is Important for Palate Formation

doi: 10.1371/journal.pone.0061653

Figure Lengend Snippet: At E15.5, palatal fusion was well under way in the wild-type embryo (+/+) (A, C, E, G). Ceacam1 −/− embryos (−/−) showed residual epithelial islands (B, D, F, H), similar to . Ceacam1 −/− MEE cells show positive Ki67 and TUNEL staining, markers for cell proliferation and apoptosis, respectively, from the anterior to the posterior part of the palate, and they express TGFβ3 at levels similar to the wild-type MEE cells. Expression levels were statistically analyzed (I, J, K); Ki67, 28.8±0.3 (+/+) (%), 30.6±2.4 (−/−) (%) (I); TUNEL, 5.0±1.4 (+/+) (%), 4.9±1.3 (−/−) (%) (J); TGFβ3, 86.9±3.7 (+/+) (%), 84.6±0.6 (−/−) (%) (K). Bars indicate s.e.m.; P = 0.52 (I), 0.50 (J), 0.50 (K), in Ceacam1 −/− palates compared to wild-type (+/+).

Article Snippet: Rabbit polyclonal antibody against TGFβ3 (Santa Cruz) or rabbit polyclonal anti-cytokeratin broad-spectrum screening antibody (Dako Cytomation) were used as the primary antibody, and goat anti-rabbit IgG (Alexa Fluor 488; Invitrogen Life Technologies), was used as the secondary antibody.

Techniques: TUNEL Assay, Staining, Expressing